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首页> 外文OA文献 >Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species
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Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

机译：使用MicroSeq D2大亚基核糖体DNA测序试剂盒来鉴定常见的具有临床意义的酵母菌种的经验

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Experience with a MicroSeq D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing kit for identification of yeast species commonly encountered in the mycology laboratory at Mayo Clinic is described here. A total of 131 isolates of yeasts recovered from clinical specimens were included in the study. Phenotypic methods used for initial identification included germ tube formation, urease production, microscopic morphological features on cornmeal agar, and an API 20C AUX system; all isolates were sequenced using a MicroSeq D2 LSU rDNA sequencing kit. Nucleic acid sequencing identified 93.9% of the isolates to the correct genus and species. A total of 100 of the isolates (representing 19 species of Candida) were sequenced, and 98% gave results concordant with identifications made by the API 20C AUX system; distance scores ranged from 0 to 1.88%, with an average value of 0.23%. Candida dubliniensis was not included in the MicroSeq database and was identified as Candida albicans. A total of 32 isolates representing 9 other genera (including Cryptococcus, Filobasidium, Kloeckera, Malassezia, Pichia, Sporidiobolus, Rhodotorula, Zygosaccharomyces, and Trichosporon) were included, and 81.3% showed concordant results when phenotypic and sequencing results were compared. Most discrepancies were attributed to the lack of inclusion of the species in the MicroSeq or API 20C AUX database. The MicroSeq D2 LSU rDNA sequencing kit appears to be accurate and useful for the identification of yeasts that might be seen in a clinical laboratory.
机译：本文介绍了使用MicroSeq D2大亚基（LSU）核糖体DNA（rDNA）测序试剂盒来鉴定Mayo诊所真菌学实验室中常见的酵母菌种的经验。从临床标本中回收的总共131种酵母菌被纳入研究。用于初步鉴定的表型方法包括胚芽管形成，脲酶生产，玉米面琼脂上的微观形态特征和API 20C AUX系统。使用MicroSeq D2 LSU rDNA测序试剂盒对所有分离物进行测序。核酸测序鉴定出93.9％的分离物属于正确的属和种。总共对100个分离株（代表19种念珠菌）进行了测序，其中98％的分离结果与API 20C AUX系统的鉴定结果相符。距离得分介于0到1.88％之间，平均值为0.23％。假丝酵母菌不包括在MicroSeq数据库中，被鉴定为白色念珠菌。总共包括32个代表9个其他属的分离株（包括隐球菌，Filobasidium，Kloeckera，Malassezia，Pichia，Sporidiobolus，Rhodotorula，Zygosaccharomyces和Trichosporon），当对表型和测序结果进行比较时，显示一致结果。大多数差异归因于MicroSeq或API 20C AUX数据库中缺少物种。 MicroSeq D2 LSU rDNA测序试剂盒看来是准确的，可用于鉴定可能在临床实验室中看到的酵母菌。

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